Product Name	Recombinant Rabbit Anti-NDUFAB1 Monoclonal Antibody
Cat No.	SLA2674M
Chinese Name	NADH氧化还原酶辅酶α1/β1重组兔单抗
Alias	ACP; ACP1; FASN2A; SDAP; 2210401F17Rik; 2310039H15Rik; 2610003B19Rik; 8kDa; 9130423F15Rik; CI-SDAP; ACPM_HUMAN; NDUFAB1; NADH-ubiquinone oxidoreductase 9.6 kDa subunit; ACPM_MOUSE; NADH:ubiquinone oxidoreductase subunit AB1; NADH dehydrogenase (ubiquinone) 1, alpha/beta subcomplex, 1 (8kD, SDAP); NADH dehydrogenase (ubiquinone) 1, alpha/beta subcomplex, 1, 8kDa; acyl carrier protein, mitochondrial; complex I SDAP subunit
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	600
React Species	Human
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Flow-Cyt (1:50-100) IP (1:20-50) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human NDUFAB1
Purification	affinity purified by Protein A
Predicted MW.	17 kDa
Observed MW.	10 kDa
Gene ID	4706
Swiss-Prot	O14561
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Carrier of the growing fatty acid chain in fatty acid biosynthesis.Accessory and non-catalytic subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which functions in the transfer of electrons from NADH to the respiratory chain (PubMed:27626371).
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with NDUFAB1 monoclonal antibody, unconjugated (SLA2674M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NDUFAB1 Monoclonal Antibody, Unconjugated (SLA2674M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NDUFAB1 Monoclonal Antibody, Unconjugated (SLA2674M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NDUFAB1 Monoclonal Antibody, Unconjugated (SLA2674M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NDUFAB1 Monoclonal Antibody, Unconjugated (SLA2674M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NDUFAB1 Monoclonal Antibody, Unconjugated (SLA2674M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.