Product Name	Recombinant Rabbit Anti-NDUFA1 Monoclonal Antibody
Cat No.	SLA2575M
Chinese Name	NADH氧化还原酶辅酶1重组兔单抗
Alias	NDUA1_HUMAN; NDUFA1; Complex I-MWFE (CI-MWFE); NADH-ubiquinone oxidoreductase MWFE subunit; CI-MWFE; MC1DN12; MWFE; ZNF183; NADH:ubiquinone oxidoreductase subunit A1; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 1 (7.5kD, MWFE); NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 1, 7.5kDa; NADH:ubiquinone oxidoreductase (complex 1); type I dehydrogenase; complex I MWFE subunit
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	5D1
React Species	Human
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human NDUFA1
Purification	affinity purified by Protein A
Predicted MW.	8 kDa
Observed MW.	10 kDa
Gene ID	4694
Swiss-Prot	O15239
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with NDUFA1 monoclonal antibody, unconjugated (SLA2575M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NDUFA1 Monoclonal Antibody, Unconjugated (SLA2575M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NDUFA1 Monoclonal Antibody, Unconjugated (SLA2575M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NDUFA1 Monoclonal Antibody, Unconjugated (SLA2575M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NDUFA1 Monoclonal Antibody, Unconjugated (SLA2575M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NDUFA1 Monoclonal Antibody, Unconjugated (SLA2575M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.

Ordering & shipping

• Minimum order: 1 unit
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