Product Name	Recombinant Rabbit Anti-PIN4 Monoclonal Antibody
Cat No.	SLA2549M
Chinese Name	PIN4重组兔单抗
Alias	PIN4_HUMAN; PIN4; Parvulin-14 (Par14 | hPar14); Parvulin-17 (Par17 | hPar17); Peptidyl-prolyl cis-trans isomerase Pin4 (PPIase Pin4); Peptidyl-prolyl cis/trans isomerase EPVH (hEPVH); Rotamase Pin4; 5.2.1.8; EPVH; PAR14; PAR17; hEPVH; hPar14; hPar17; peptidylprolyl cis/trans isomerase, NIMA-interacting 4; protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting, 4 (parvulin); peptidylprolyl cis/trans isomerase NIMA-interacting, 4; parvulin
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	3A11
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Flow-Cyt (1:50-100) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human PIN4
Purification	affinity purified by Protein A
Predicted MW.	14 kDa
Observed MW.	13 kDa
Subcellular Location	N/A
Gene ID	5303
Swiss-Prot	Q9Y237
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Isoform 1 is involved as a ribosomal RNA processing factor in ribosome biogenesis. Binds to tightly bent AT-rich stretches of double-stranded DNA.Isoform 2 binds to double-stranded DNA.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with PIN4 monoclonal antibody, unconjugated (SLA2549M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PIN4 Monoclonal Antibody, Unconjugated (SLA2549M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 3. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PIN4 Monoclonal Antibody, Unconjugated (SLA2549M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 4. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PIN4 Monoclonal Antibody, Unconjugated (SLA2549M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 5. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PIN4 Monoclonal Antibody, Unconjugated (SLA2549M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 6. The HeLa (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-PIN4 antibody (SLA2549M,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.