Product Name	SEMA4D | CD100/SEMAJ Recombinant Rabbit mAb
Cat No.	SLM-62573R
Chinese Name	跨膜蛋白SEMA4D重组兔单抗
Alias	A8; BB18; C9orf164; CD100; COLL4; GR3; M-sema-G; SEMAJ; coll-4; SEM4D_HUMAN; SEMA4D; semaphorin 4D; chromosome 9 open reading frame 164; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D; M-sema G
Host Species	Rabbit
Isotype	IgG
Clonality	Recombinant
Clone No.	9H4
React Species	Human
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) ICC/IF (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human Semaphorin 4D
Purification	affinity purified by Protein A
Concentration
Predicted MW.	96 kDa
Observed MW.	150 kDa
Subcellular Location	Membrane
Gene ID	10507
Swiss-Prot	Q92854
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Cell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling.Regulates GABAergic synapse development.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with SEMA4D monoclonal antibody, unconjugated (SLM-62573R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SEMA4D Monoclonal Antibody, Unconjugated (SLM-62573R) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SEMA4D Monoclonal Antibody, Unconjugated (SLM-62573R) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SEMA4D Monoclonal Antibody, Unconjugated (SLM-62573R) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SEMA4D Monoclonal Antibody, Unconjugated (SLM-62573R) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SEMA4D Monoclonal Antibody, Unconjugated (SLM-62573R) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.