Product Name	Recombinant Rabbit Anti-TMEFF2 Monoclonal Antibody
Cat No.	SLA2226M
Chinese Name	增生性息肉蛋白1重组兔单抗
Alias	CT120.2; HPP1; TENB2; TPEF; TR; TR-2; 4832418D20Rik; 7630402F16Rik; TEFF2_BOVIN; TMEFF2; Transmembrane protein with EGF-like and two follistatin-like domains; TEFF2_HUMAN; Hyperplastic polyposis protein 1; TEFF2_MOUSE; transmembrane protein with EGF like and two follistatin like domains 2; transmembrane protein with EGF-like and two follistatin-like domains 2; transmembrane protein TENB2; tomoregulin; cancer/testis antigen family 120, member 2
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	6B12
React Species	Human
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Flow-Cyt (1:50-100) ICC/IF (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human TR 2
Purification	affinity purified by Protein A
Predicted MW.	41 kDa
Observed MW.	44 kDa
Subcellular Location	N/A
Gene ID	23671
Swiss-Prot	Q9UIK5
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	May be a survival factor for hippocampal and mesencephalic neurons. The shedded form up-regulates cancer cell proliferation, probably by promoting ERK1/2 phosphorylation.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with TMEFF2 monoclonal antibody, unconjugated (SLA2226M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TMEFF2 Monoclonal Antibody, Unconjugated (SLA2226M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TMEFF2 Monoclonal Antibody, Unconjugated (SLA2226M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TMEFF2 Monoclonal Antibody, Unconjugated (SLA2226M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TMEFF2 Monoclonal Antibody, Unconjugated (SLA2226M) at 1:200 overnight at 4°C.Followed by conjugated Goat Anti-Ratbbit IgG antibody (red, SL0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei	Figure 6. The SH-SY5Y (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-TMEFF2 antibody (SLA2226M,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488 (SL60295G-BF488): 1 μg/test.Blank control (black): PBS. Acquisition of 20,000 events was performed

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.