Product Name	Recombinant Rabbit Anti-MKI67 Monoclonal Antibody
Cat No.	SLA0345M
Chinese Name	Ki67单克隆抗体
Alias	KIA; MIB-; MIB-1; PPP1R105; KI67_HUMAN; MKI67; Antigen identified by monoclonal antibody Ki-67 (Antigen KI-67 | Antigen Ki67); marker of proliferation Ki-67; antigen identified by monoclonal antibody Ki-67; protein phosphatase 1, regulatory subunit 105; Molecular Immunology Borstel antibody 1
Host Species	Mouse
Isotype	IgG
Clonality	Monoclonal
Clone No.	2B10
React Species	Human
Applications	IHC-P (1:1000-5000) IHC-F (1:1000-5000) IF (1:1000-5000) Flow-Cyt (1ug/Test) ICC/IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	KLH conjugated synthetic peptide derived from human Ki67
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	358 kDa
Observed MW.
Subcellular Location	Nucleus
Gene ID	4288
Swiss-Prot	P46013
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ki-67 Monoclonal Antibody, Unconjugated (SLA0345M) at 1:1000 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Serous ovarian cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ki-67 Monoclonal Antibody, Unconjugated (SLA0345M) at 1:1000 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ki-67 Monoclonal Antibody, Unconjugated (SLA0345M) at 1:1000 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ki-67 Monoclonal Antibody, Unconjugated (SLA0345M) at 1:1000 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining
Figure 5. (Negative control) Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ki-67 Monoclonal Antibody, Unconjugated (SLA0345M) at 1:1000 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining	Figure 6. The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Mouse Anti-Ki-67 antibody (SLM-258161M): 1 μg/10^6 cells; Isotype Control (orange): Mouse IgG (SL0296P). Blank control (black): PBS. Acquisition of 20,000 events was performed
Figure 7. 4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Ki-67) monoclonal Antibody, unconjugated (SLM-0061R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Mouse IgG antibody (green, SL60296G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.