Product Name	Recombinant Rabbit Anti-TFAP2A Monoclonal Antibody
Cat No.	SLA2171M
Chinese Name	转录激活蛋白2α/TFAP2α/AP-2α重组兔单抗
Alias	AP-2; AP-2alpha; AP2TF; BOFS; TFAP2; AP2alpha; Ap-2 (a); Ap2; Tcfap2a; AP2A_HUMAN; TFAP2A; AP2-alpha; AP-2 transcription factor; Activating enhancer-binding protein 2-alpha; Activator protein 2 (AP-2); AP2A_MOUSE; AP2A_RAT; transcription factor AP-2 alpha; transcription factor AP-2 alpha (activating enhancer-binding protein 2 alpha); transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha)
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	5B12
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:50-200) IHC-F (1:50-200) IF (1:50-200) IP (1:20-50) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human AP 2 alpha
Purification	affinity purified by Protein A
Predicted MW.	48 kDa
Observed MW.	48 kDa
Gene ID	7020
Swiss-Prot	P05549
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with AP2 alpha monoclonal antibody, unconjugated (SLA2171M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with AP2 alpha Monoclonal Antibody, Unconjugated (SLA2171M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with AP2 alpha Monoclonal Antibody, Unconjugated (SLA2171M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with AP2 alpha Monoclonal Antibody, Unconjugated (SLA2171M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Mouse Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with AP2 alpha Monoclonal Antibody, Unconjugated (SLA2171M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with AP2 alpha Monoclonal Antibody, Unconjugated (SLA2171M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.

Ordering & shipping

• Minimum order: 1 unit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
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• Price: USD, per unit (25ul / 50ul / 100ul)