Product Name	Recombinant Rabbit Anti-SPRY2 Monoclonal Antibody
Cat No.	SLA1817M
Chinese Name	快速发育生长因子同源蛋白2重组兔单抗
Alias	IGAN3; hSPRY2; sprouty2; SPY2_HUMAN; SPRY2; Spry-2; SPY2_MOUSE; sprouty RTK signaling antagonist 2; sprouty (Drosophila) homolog 2; sprouty homolog 2 (Drosophila)
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	12C9
React Species	Human,Rat
Applications	WB (1:1000-2000) IHC-P (1:100-200) IHC-F (1:100-200) IF (1:50-200) Flow-Cyt (1:50-100) IP (1:20-50) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human Spry 2
Purification	affinity purified by Protein A
Predicted MW.	35 kDa
Observed MW.	40 kDa
Subcellular Location	N/A
Gene ID	10253
Swiss-Prot	O43597
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Antagonist of fibroblast growth factor (FGF) pathways via inhibition of FGF-mediated phosphorylation of ERK1/2.Thereby acts as an antagonist of FGF-induced retinal lens fiber differentiation, may inhibit limb bud outgrowth and may negatively modulate respiratory organogenesis.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Sprouty 2 Monoclonal Antibody, Unconjugated (SLA1817M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Sprouty 2 Monoclonal Antibody, Unconjugated (SLA1817M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Sprouty 2 Monoclonal Antibody, Unconjugated (SLA1817M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Sprouty 2 Monoclonal Antibody, Unconjugated (SLA1817M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 5. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Sprouty 2 Monoclonal Antibody, Unconjugated (SLA1817M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Ratbbit IgG antibody (Red, SL0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei	Figure 6. The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-Sprouty 2 antibody (SLA1817M,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.

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