Product Name	Recombinant Rabbit Anti-DTL Monoclonal Antibody
Cat No.	SLA1742M
Chinese Name	维甲酸调节核基质相关蛋白重组兔单抗
Alias	DTL_HUMAN; DTL; DDB1- and CUL4-associated factor 2; Lethal(2) denticleless protein homolog; Retinoic acid-regulated nuclear matrix-associated protein; CDT2; CDW1; DCAF2; L2DTL; RAMP; 2810047L02Rik; 5730564G15Rik; RGD1310439; DTL_MOUSE; Meth A retinoic acid-regulated nuclear matrix-associated protein (Meth A RAMP); DTL_CHICK; denticleless E3 ubiquitin protein ligase adapter; denticleless E3 ubiquitin protein ligase homolog; denticleless homolog (Drosophila); denticleless E3 ubiquitin protein ligase homolog (Drosophila); RA regulated nuclear matrix associated protein; DDB1 and CUL4 associated factor 2
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	20C2
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Flow-Cyt (1:50-100) ICC/IF (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human CDT2
Purification	affinity purified by Protein A
Predicted MW.	79 kDa
Observed MW.	87 kDa
Gene ID	51514
Swiss-Prot	Q9NZJ0
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1, CDKN1A/p21(CIP1), FBH1, KMT5A and SDE2.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with CDT2 monoclonal antibody, unconjugated (SLA1742M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CDT2 Monoclonal Antibody, Unconjugated (SLA1742M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Prostate Tumor; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CDT2 Monoclonal Antibody, Unconjugated (SLA1742M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CDT2 Monoclonal Antibody, Unconjugated (SLA1742M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CDT2 Monoclonal Antibody, Unconjugated (SLA1742M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CDT2 Monoclonal Antibody, Unconjugated (SLA1742M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.