DESCRIPTION

SLC27A6, also known as FATP6, ACSVL2, FACVL2, belongs to the ATP-dependent AMP-binding enzyme family. SLC27A6 mediates the import of long-chain fatty acids (LCFA) into the cell by facilitating their transport at the plasma membrane. SLC27A6 plays a pivotal role in regulating available LCFA substrates from exogenous sources in tissues undergoing high levels of beta-oxidation. SLC27A6 is strongly expressed in heart and localizes to cardiac myocytes. SLC27A6 is expressed at moderate levels in placenta, testis, and adrenal glands.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	8	29.64	2.13	7.18
2	8	7.02	0.39	5.60
3	8	3.79	0.16	4.12

Inter-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	16	30.31	2.84	9.38
2	16	7.21	0.42	5.89
3	16	3.87	0.19	4.95

RECOVERY

The recovery of human SLC27A6 spiked to three different levels throughout the range of the assay in cell lysate was evaluated.

Sample Type		Average% of Expected	Range (%)
Cell lysate	1:128	102	92-108

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The cell lysate was initially diluted 1:8.)

		Cell lysate
1:2	Average% of Expected	100
	Range (%)	-
1:4	Average% of Expected	102
	Range (%)	94-113
1:8	Average% of Expected	106
	Range (%)	97-121
1:16	Average% of Expected	102
	Range (%)	84-130

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human SLC27A6 Sandwich ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
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• Price: USD, per kit (48T / 96T options available)