Product Name	Recombinant Rabbit Anti-SHH Monoclonal Antibody
Cat No.	SLA1233M
Chinese Name	Shh信号转导通路膜蛋白受体重组兔单抗
Alias	HHG1; HLP3; HPE3; MCOPCB5; SMMCI; ShhNC; TPT; TPTPS; SHH_HUMAN; SHH; HHG-1; Shh unprocessed N-terminal signaling and C-terminal autoprocessing domains (ShhNC); 3.1.-.-; sonic hedgehog signaling molecule; sonic hedgehog (Drosophila) homolog; SHH signaling molecule
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	18F7
React Species	Human
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Flow-Cyt (1:50-100) ICC/IF (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human Sonic hedgehog protein
Purification	affinity purified by Protein A
Predicted MW.	50 kDa
Observed MW.	50,27 kDa
Gene ID	6469
Swiss-Prot	Q15465
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	The C-terminal part of the sonic hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity (By similarity).Both activities result in the cleavage of the full-length protein into two parts (ShhN and ShhC) followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated ShhN (By similarity).Both activities occur in the reticulum endoplasmic (By similarity).
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Shh Monoclonal Antibody, Unconjugated(SLA1233M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Pancreatic Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Shh Monoclonal Antibody, Unconjugated(SLA1233M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Shh Monoclonal Antibody, Unconjugated (SLA1233M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Shh Monoclonal Antibody, Unconjugated (SLA1233M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Shh Monoclonal Antibody, Unconjugated (SLA1233M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Shh Monoclonal Antibody, Unconjugated (SLA1233M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.

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