Product Name	Recombinant Rabbit Anti-CPT1A Monoclonal Antibody
Cat No.	SLA1154M
Chinese Name	肉毒碱棕榈酰基转移酶1A单克隆抗体
Alias	CPT I; CPT1; CPT1-L; CPTI-L; L-CPT1; C730027G07; CPTI; CPT-Ia; CPT1A_HUMAN; CPT1A; Carnitine O-palmitoyltransferase I, liver isoform (CPT I | CPTI-L); Carnitine palmitoyltransferase 1A; Succinyltransferase CPT1A; 2.3.1.21; CPT1A_MOUSE; Cpt-1; CPT1A_RAT; carnitine palmitoyltransferase 1A (liver)
Host Species	Mouse
Isotype	IgG2b,k
Clonality	Monoclonal
Clone No.	3E4
React Species	Human,Mouse,Rat
Applications	IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	KLH conjugated synthetic peptide derived from human CPT1
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	86 kDa
Subcellular Location	Cytoplasm
Gene ID	1374
Swiss-Prot	P50416
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	The mitochondrial oxidation of long-chain fatty acids is nitiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CPT1A Monoclonal Antibody, Unconjugated (SLA1154M) at 1:300 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CPT1A Monoclonal Antibody, Unconjugated (SLA1154M) at 1:300 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CPT1A Monoclonal Antibody, Unconjugated (SLA1154M) at 1:300 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CPT1A Monoclonal Antibody, Unconjugated (SLA1154M) at 1:300 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CPT1A Monoclonal Antibody, Unconjugated (SLA1154M) at 1:300 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CPT1A Monoclonal Antibody, Unconjugated (SLA1154M) at 1:300 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining.
Figure 7. Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CPT1A Monoclonal Antibody, Unconjugated (SLA1154M) at 1:300 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.