Product Name	Recombinant Rabbit Anti-SP7 Monoclonal Antibody
Cat No.	SLA0960M
Chinese Name	成骨相关转录因子重组兔单抗
Alias	OI11; OI12; OSX; osterix; 6430578P22Rik; C22; SP7_HUMAN; SP7; Zinc finger protein osterix; Q5RM08_MOUSE; SP7_MOUSE; Q6IMK1_RAT; Sp7 transcription factor
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	3A5
React Species	Human,Mouse,Rat
Applications	WB (1:500-1000) IHC-P (1:200-1000) IHC-F (1:200-1000) IF (1:200-1000) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	Recombinant human Sp7 protein
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	45 kDa
Observed MW.	45 kDa
Subcellular Location	Nucleus
Gene ID	121340
Swiss-Prot	Q8TDD2
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	This gene encodes a member of the Sp subfamily of Sp/XKLF transcription factors. Sp family proteins are sequence-specific DNA-binding proteins characterized by an amino-terminal trans-activation domain and three carboxy-terminal zinc finger motifs. This protein is a bone specific transcription factor and is required for osteoblast differentiation and bone formation.[provided by RefSeq, Jul 2010]
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with SP7 | Osterix monoclonal antibody, unconjugated (SLA0960M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Embryo; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SP7 | Osterix Monoclonal Antibody, Unconjugated (SLA0960M) at 1:800 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Mouse Embryo; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SP7 | Osterix Monoclonal Antibody, Unconjugated (SLA0960M) at 1:800 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Rat Embryo; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SP7 | Osterix Monoclonal Antibody, Unconjugated (SLA0960M) at 1:800 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Mouse cartilage; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SP7 | Osterix Monoclonal Antibody, Unconjugated (SLA0960M) at 1:800 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Rat cartilage; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SP7 | Osterix Monoclonal Antibody, Unconjugated (SLA0960M) at 1:800 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 7. Paraformaldehyde-fixed, paraffin embedded Mouse bone; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SP7 | Osterix Monoclonal Antibody, Unconjugated (SLA0960M) at 1:800 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.