Product Name	Recombinant Rabbit Anti-OCLN Monoclonal Antibody
Cat No.	SLA0959M
Chinese Name	紧密连接蛋白重组兔单抗
Alias	Ocl; BLCPMG; PPP1R115; PTORCH1; OCLN_CANLF; OCLN; OCLN_HUMAN; OCLN_MOUSE; OCLN_RAT; occludin; tight junction protein occludin TM4 minus; phosphatase 1, regulatory subunit 115
Host Species	Rabbit
Clonality	Monoclonal
Clone No.	8F3
React Species	Human,Mouse,Rat
Applications	WB (1:2000-10000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) ELISA (1:5000-20000) IP (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	Recombinant human Occludin protein
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	57 kDa
Subcellular Location	Membrane
Gene ID	100506658
Swiss-Prot	Q61146
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	This gene encodes an integral membrane protein which is located at tight junctions. This protein may be involved in the formation and maintenance of the tight junction. The possibility of several alternatively spliced products has been suggested but the full nature of these products has not been described. [provided by RefSeq].
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OCLN | Occludin Monoclonal Antibody, Unconjugated (SLA0959M) at 1:200 overnight at 4°C, followed by conjugation to SL0295G-HRP and DAB (C-0010) staining.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OCLN | Occludin Monoclonal Antibody, Unconjugated (SLA0959M) at 1:200 overnight at 4°C, followed by conjugation to SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Mouse Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OCLN | Occludin Monoclonal Antibody, Unconjugated (SLA0959M) at 1:200 overnight at 4°C, followed by conjugation to SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Rat Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OCLN | Occludin Monoclonal Antibody, Unconjugated (SLA0959M) at 1:200 overnight at 4°C, followed by conjugation to SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OCLN | Occludin Monoclonal Antibody, Unconjugated (SLA0959M) at 1:200 overnight at 4°C, followed by conjugation to SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OCLN | Occludin Monoclonal Antibody, Unconjugated (SLA0959M) at 1:200 overnight at 4°C, followed by conjugation to SL0295G-HRP and DAB (C-0010) staining.
Figure 7. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OCLN | Occludin Monoclonal Antibody, Unconjugated (SLA0959M) at 1:200 overnight at 4°C, followed by conjugation to SL0295G-HRP and DAB (C-0010) staining.	Figure 8. Paraformaldehyde-fixed, paraffin embedded Mouse Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OCLN | Occludin Monoclonal Antibody, Unconjugated (SLA0959M) at 1:200 overnight at 4°C, followed by conjugation to SL0295G-HRP and DAB (C-0010) staining.
Figure 9. Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OCLN | Occludin Monoclonal Antibody, Unconjugated (SLA0959M) at 1:200 overnight at 4°C, followed by conjugation to SL0295G-HRP and DAB (C-0010) staining.	Figure 10. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OCLN | Occludin Monoclonal Antibody, Unconjugated (SLA0959M) at 1:200 overnight at 4°C, followed by conjugation to SL0295G-HRP and DAB (C-0010) staining.
Figure 11. Paraformaldehyde-fixed, paraffin embedded Human Clear cell carcinoma of ovary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OCLN | Occludin Monoclonal Antibody, Unconjugated (SLA0959M) at 1:200 overnight at 4°C, followed by conjugation to SL0295G-HRP and DAB (C-0010) staining.	Figure 12. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OCLN | Occludin Monoclonal Antibody, Unconjugated (SLA0959M) at 1:200 overnight at 4°C, followed by conjugation to SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.