DESCRIPTION

SOX4 is a transcription factor belonging to the SOX family, which is crucial for embryonic development and cell fate determination. It is located on chromosome 6p22.3 in humans and encodes a protein of 474 amino acids with three distinguishable domains: a high mobility group (HMG) box, a glycine-rich region, and a serine-rich region. This protein regulates gene expression by binding to DNA and interacting with other proteins, thereby influencing cell proliferation, differentiation, and apoptosis. SOX4 has been implicated in various diseases, including cancer and atherosclerosis, due to its role in promoting cell proliferation and survival. For instance, SOX4 is highly expressed in several cancers, such as non-small cell lung cancer (NSCLC), and is associated with poor prognosis. It can induce epithelial-mesenchymal transition (EMT), enhancing cancer cell invasion and metastasis. Additionally, SOX4 contributes to atherosclerosis by regulating endothelial cell function. Its diverse functions and involvement in multiple signaling pathways make SOX4 a significant focus in both basic research and clinical studies.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	1795.1	71.7	4.0
2	8	387.3	19.4	5.0
3	8	100.1	4.8	4.8

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	1,791.7	72.6	4.0
2	16	383.5	17.6	4.6
3	16	100.5	6.2	6.2

RECOVERY

The recovery of human SOX4 spiked to three different levels throughout the range of the assay in cell lysate was evaluated.

Sample Type		Average% of Expected	Range (%)
Cell lysate	1:16	109	109-110

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The cell lysate was initially diluted 1:8.)

		Cell lysate
1:2	Average% of Expected	100
	Range (%)	-
1:4	Average% of Expected	116
	Range (%)	109-123
1:8	Average% of Expected	129
	Range (%)	127-130
1:16	Average% of Expected	110
	Range (%)	106-115

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human SOX4 Sandwich ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)