DESCRIPTION

SORBS2 also known as ARGBP2, has three C-terminal SH3 domains and an N-terminal sorbin homology (SoHo) domain that interacts with lipid raft proteins. SORBS2 is widely expressed in human tissues and extremely abundant in the heart. In epithelial cells SORBS2 is located in stress fibers and the nucleus; In cardiac muscle cells. SORBS2 is situated in the Z-disks of sarcomeres. The subcellular localization suggests that SORBS2 functions as an adapter protein to assemble signaling complexes in stress fibers, and may influence the contractile or elastic properties of cardiac sarcomeres. The expression of SORBS2 was downregulated in pancreatic adenocarcinomas and is linked to cell adhesion and migration.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 20 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 24 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	20	2,283.4	105.0	4.6
2	20	527.8	23.4	4.4
3	20	148.3	9.4	6.3

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	24	2,267.2	131.3	5.8
2	24	514.0	24.2	4.7
3	24	143.7	6.5	4.5

RECOVERY

The recovery of human SORBS2 spiked to three different levels throughout the range of the assay in human serum was evaluated.

Sample Type		Average% of Expected	Range (%)
Human serum	1:2	81	76-86
	1:4	84	72-101

LINEARITY

To assess the linearity of the assay, human serum samples were spiked with high concentrations of human SORBS2 and diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay.

		Human serum
1:2	Average% of Expected	76
	Range (%)	73-79
1:4	Average% of Expected	81
	Range (%)	79-82
1:8	Average% of Expected	79
	Range (%)	78-80
1:16	Average% of Expected	76
	Range (%)	73-80

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human SORBS2 produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
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• Price: USD, per kit (48T / 96T options available)