Product Name	Recombinant Rabbit Anti-BIRC3 Monoclonal Antibody
Cat No.	SLA1245M
Chinese Name	凋亡抑制因子2重组兔单抗
Alias	AIP1; API2; CIAP2; HAIP1; HIAP1; IAP-1; MALT2; MIHC; RNF49; c-IAP2; Api1; HIAP2; IAP1; IAP2; MIAP1; MIAP2; MIHB; cIAP-1; cIAP-2; cIAP1; Birc2; BIRC3_HUMAN; BIRC3; Apoptosis inhibitor 2 (API2); Cellular inhibitor of apoptosis 2 (C-IAP2); IAP homolog C; Inhibitor of apoptosis protein 1 (hIAP-1 | hIAP1); RING finger protein 49; RING-type E3 ubiquitin transferase BIRC3; TNFR2-TRAF-signaling complex protein 1; 2.3.2.27; BIRC3_MOUSE; Inhibitor of apoptosis protein 1 (mIAP1); baculoviral IAP repeat containing 3; baculoviral IAP repeat-containing 3; apoptosis inhibitor 2; TNFR2-TRAF signaling complex protein; mammalian IAP homolog C; inhibitor of apoptosis protein 1
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	6C19
React Species	Human
Applications	WB (1:500-2000) IHC-P (1:50-200) IHC-F (1:50-200) IF (1:50-200) ICC/IF (1:50-100) IP (1:20-50) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human BIRC3
Purification	affinity purified by Protein A
Predicted MW.	68 kDa
Observed MW.	68 kDa
Gene ID	330
Swiss-Prot	Q13489
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, mitogenic kinase signaling and cell proliferation, as well as cell invasion and metastasis. Acts as an E3 ubiquitin-protein ligase regulating NF-kappa-B signaling and regulates both canonical and non-canonical NF-kappa-B signaling by acting in opposite directions: acts as a positive regulator of the canonical pathway and suppresses constitutive activation of non-canonical NF-kappa-B signaling.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with API2/BIRC3 monoclonal antibody, unconjugated (SLA1245M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with API2/BIRC3 Monoclonal Antibody, Unconjugated(SLA1245M) at 1:100 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Rhabdomyosarcoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with API2/BIRC3 Monoclonal Antibody, Unconjugated(SLA1245M) at 1:100 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with API2/BIRC3 Monoclonal Antibody, Unconjugated(SLA1245M) at 1:100 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with API2/BIRC3 Monoclonal Antibody, Unconjugated(SLA1245M) at 1:100 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with API2/BIRC3 Monoclonal Antibody, Unconjugated(SLA1245M) at 1:100 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 7. Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with API2/BIRC3 Monoclonal Antibody, Unconjugated(SLA1245M) at 1:100 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.