Product Name	Recombinant Rabbit Anti-CDKN1C Monoclonal Antibody
Cat No.	SLA1262M
Chinese Name	周期蛋白依赖激酶抑制因子1C重组兔单抗
Alias	BWCR; BWS; KIP2; WBS; p57; p57Kip2; CDKI; p57(kip2); CDN1C_HUMAN; CDKN1C; Cyclin-dependent kinase inhibitor p57; CDN1C_MOUSE; E9PTV7_RAT; Q69DC0_RAT; cyclin dependent kinase inhibitor 1C; Beckwith-Wiedemann syndrome; cyclin-dependent kinase inhibitor 1C (p57, Kip2)
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	4E7
React Species	Human,Mouse,Rat
Applications	WB (1:1000-2000) IHC-P (1:100-200) IHC-F (1:100-200) IF (1:100-200) IP (1:20-50) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human p57 Kip2
Purification	affinity purified by Protein A
Predicted MW.	32 kDa
Observed MW.	50 kDa
Gene ID	1028
Swiss-Prot	P49918
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Potent tight-binding inhibitor of several G1 cyclin/CDK complexes (cyclin E-CDK2, cyclin D2-CDK4, and cyclin A-CDK2) and, to lesser extent, of the mitotic cyclin B-CDC2. Negative regulator of cell proliferation. May play a role in maintenance of the non-proliferative state throughout life.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with p57 Kip2/Cdkn1c Monoclonal Antibody, Unconjugated(SLA1262M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with p57 Kip2/Cdkn1c Monoclonal Antibody, Unconjugated(SLA1262M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with p57 Kip2/Cdkn1c Monoclonal Antibody, Unconjugated(SLA1262M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Mouse Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with p57 Kip2/Cdkn1c Monoclonal Antibody, Unconjugated(SLA1262M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Rat Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with p57 Kip2/Cdkn1c Monoclonal Antibody, Unconjugated(SLA1262M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with p57 Kip2/Cdkn1c Monoclonal Antibody, Unconjugated(SLA1262M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 7. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with p57 Kip2/Cdkn1c Monoclonal Antibody, Unconjugated(SLA1262M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.