Product Name	Recombinant Rabbit Anti-MEK3&MEK6 Monoclonal Antibody
Cat No.	SLA2979M
Chinese Name	丝裂原活化蛋白激酶MKK3/MKK6重组兔单抗
Alias	MAPKK3; MEK3; MKK3; PRKMK3; SAPKK-2; SAPKK2; MAPKK 3; MKK3b; MP2K3_HUMAN; MAP2K3; MAP kinase kinase 3; MAPK/ERK kinase 3 (MEK 3); Stress-activated protein kinase kinase 2 (SAPK kinase 2 | SAPKK-2 | SAPKK2); 2.7.12.2; SKK2; MP2K3_MOUSE; mitogen-activated protein kinase kinase 3; MAPK/ERK kinase 3; dual specificity mitogen activated protein kinase kinase 3; stress-activated protein kinase kinase 2
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	10E1
React Species	Human
Applications	WB (1:500-2000) IHC-P (1:50-200) IHC-F (1:50-200) IF (1:50-200) Flow-Cyt (1:50-100) ICC/IF (1:50-200) IP (1:20-50) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human MEK3
Purification	affinity purified by Protein A
Predicted MW.	39,37 kDa
Observed MW.	39, 37 kDa
Subcellular Location	N/A
Gene ID	5606
Swiss-Prot	P46734
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with MEK3/MEK6 monoclonal antibody, unconjugated (SLA2979M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MEK3/MEK6 Monoclonal Antibody, Unconjugated (SLA2979M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MEK3/MEK6 Monoclonal Antibody, Unconjugated (SLA2979M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MEK3/MEK6 Monoclonal Antibody, Unconjugated (SLA2979M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MEK3/MEK6 Monoclonal Antibody, Unconjugated (SLA2979M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MEK3/MEK6 Monoclonal Antibody, Unconjugated (SLA2979M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 7. The HeLa (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-MEK3/MEK6 antibody (SLA2979M,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed	Figure 8. 4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (MEK3/MEK6) monoclonal Antibody, unconjugated (SLA2979M) 1:100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, SL60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.