Product Name	Recombinant Rabbit Anti-GYS1 Monoclonal Antibody
Cat No.	SLA2971M
Chinese Name	葡萄糖合成酶1重组兔单抗
Alias	GSY; GYS; Gys3; MGS; GYS1_HUMAN; GYS1; Glycogen synthase 1; 2.4.1.11; Q8VEB0_MOUSE; GYS1_MOUSE; GYS1_RAT; glycogen synthase 1 (muscle)
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	5G11
React Species	Human,Mouse,Rat
Applications	WB (1:1000-5000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Flow-Cyt (1:50-100) IP (1:20-50) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human Glycogen synthase
Purification	affinity purified by Protein A
Predicted MW.	84 kDa
Observed MW.	95 kDa
Subcellular Location	Cytoplasm
Gene ID	2997
Swiss-Prot	P13807
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Glycogen synthase participates in the glycogen biosynthetic process along with glycogenin and glycogen branching enzyme. Extends the primer composed of a few glucose units formed by glycogenin by adding new glucose units to it. In this context, glycogen synthase transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Paraformaldehyde-fixed, paraffin embedded Mouse Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Glycogen synthase 1 Monoclonal Antibody, Unconjugated (SLA2971M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 2. Paraformaldehyde-fixed, paraffin embedded Rat Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Glycogen synthase 1 Monoclonal Antibody, Unconjugated (SLA2971M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Glycogen synthase 1 Monoclonal Antibody, Unconjugated (SLA2971M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 4. Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Glycogen synthase 1 Monoclonal Antibody, Unconjugated (SLA2971M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 5. Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Glycogen synthase 1 Monoclonal Antibody, Unconjugated (SLA2971M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 6. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Glycogen synthase 1 Monoclonal Antibody, Unconjugated (SLA2971M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 7. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Glycogen synthase 1 Monoclonal Antibody, Unconjugated (SLA2971M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 8. The U-87 MG (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Cells stained with Primary Antibody for 30 min at room temperature,followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-Glycogen synthase 1 antibody (SLA2971M,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.

Ordering & shipping

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