Product Name	Recombinant Rabbit Anti-SREBF1 Monoclonal Antibody
Cat No.	SLA0949M
Chinese Name	胆固醇调节元件结合蛋白1重组兔单抗
Alias	HMD; IFAP2; SREBP1; bHLHd1; ADD1; ADD-1; SREBP-1; SREBP-1c; SRBP1_HUMAN; SREBF1; Class D basic helix-loop-helix protein 1 (bHLHd1); Sterol regulatory element-binding transcription factor 1; SRBP1_MOUSE; SRBP1_RAT; Adipocyte determination- and differentiation-dependent factor 1 (ADD1); sterol regulatory element binding transcription factor 1; Sterol regulatory element-binding protein 1
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	8G7
React Species	Human
Applications	IHC-P (1:200-1000) IHC-F (1:200-1000) IF (1:200-1000) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	Recombinant human SREBF1 protein
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	122 kDa
Subcellular Location	Nucleus
Gene ID	6720
Swiss-Prot	P36956
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	This gene encodes a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. The protein is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SREBF1/SREBP1 Monoclonal Antibody, Unconjugated (SLA0949M) at 1:1000 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SREBF1/SREBP1 Monoclonal Antibody, Unconjugated (SLA0949M) at 1:1000 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SREBF1/SREBP1 Monoclonal Antibody, Unconjugated (SLA0949M) at 1:1000 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SREBF1 | SREBP1 Monoclonal Antibody, Unconjugated (SLA0949M) at 1:800 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SREBF1 | SREBP1 Monoclonal Antibody, Unconjugated (SLA0949M) at 1:800 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SREBF1/SREBP1 Monoclonal Antibody, Unconjugated (SLA0949M) at 1:1000 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 7. Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SREBF1/SREBP1 Monoclonal Antibody, Unconjugated (SLA0949M) at 1:1000 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 8. Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SREBF1/SREBP1 Monoclonal Antibody, Unconjugated (SLA0949M) at 1:1000 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 9. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SREBF1/SREBP1 Monoclonal Antibody, Unconjugated (SLA0949M) at 1:1000 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 10. Paraformaldehyde-fixed, paraffin embedded Human Adrenal Gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SREBF1 | SREBP1 Monoclonal Antibody, Unconjugated (SLA0949M) at 1:800 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.