Product Name	Recombinant Rabbit Anti-CASP1 Monoclonal Antibody
Cat No.	SLA0991M
Chinese Name	天冬氨酸-胱氨酸特异性蛋白酶家族重组兔单抗
Alias	ICE; IL1BC; P45; CASP1_HUMAN; CASP1; CASP-1; Interleukin-1 beta convertase (IL-1BC); Interleukin-1 beta-converting enzyme (ICE | IL-1 beta-converting enzyme); 3.4.22.36; IL1BCE; CASP1_MOUSE; CASP1_RAT; caspase 1; caspase 1, apoptosis-related cysteine protease (interleukin 1, beta, convertase); caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase); caspase 1, apoptosis-related cysteine peptidase; caspase-1; interleukin 1, beta, convertase
Host Species	Rabbit
Isotype	IgG, k
Clonality	Monoclonal
Clone No.	7A8
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:200-800) IHC-F (1:200-800) IF (1:200-800) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	Recombinant human Caspase1 protein
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	45 kDa
Observed MW.	45-47 kDa
Gene ID	834
Swiss-Prot	P29466
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing of this gene results in five transcript variants encoding distinct isoforms. [provided by RefSeq].
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with Caspase-1 monoclonal antibody, unconjugated (SLA0991M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Caspase-1 Monoclonal Antibody, Unconjugated (SLA0991M) at 1:800 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Caspase-1 Monoclonal Antibody, Unconjugated (SLA0991M) at 1:800 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Caspase-1 Monoclonal Antibody, Unconjugated (SLA0991M) at 1:800 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Caspase-1 Monoclonal Antibody, Unconjugated (SLA0991M) at 1:800 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Caspase-1 Monoclonal Antibody, Unconjugated (SLA0991M) at 1:800 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 7. Paraformaldehyde-fixed, paraffin embedded Mouse Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Caspase-1 Monoclonal Antibody, Unconjugated (SLA0991M) at 1:800 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 8. Paraformaldehyde-fixed, paraffin embedded Rat Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Caspase-1 Monoclonal Antibody, Unconjugated (SLA0991M) at 1:800 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 9. Paraformaldehyde-fixed, paraffin embedded Mouse Lymph; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Caspase-1 Monoclonal Antibody, Unconjugated (SLA0991M) at 1:800 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 10. Paraformaldehyde-fixed, paraffin embedded Human Lymph; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Caspase-1 Monoclonal Antibody, Unconjugated (SLA0991M) at 1:800 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 11. Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Caspase-1 Monoclonal Antibody, Unconjugated (SLA0991M) at 1:800 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 12. Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Caspase-1 Monoclonal Antibody, Unconjugated (SLA0991M) at 1:800 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.