Summary:

Purpose

Our Quick Step Monkey Interleukin 12 (IL-12) ELISA Kit is to assay IL-12 levels in Monkey serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to IL-12. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for IL-12 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain IL-12 and HRP conjugated IL-12 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of IL-12. You can calculate the concentration of IL-12 in the samples by comparing the OD of the samples to the standard curve.

Typical Data

The standard curve of QS0021Mk is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Standard	Concentration	OD Value	Average OD Value
Blank Well	0pg/ml	0.044	0.0480
Blank Well	0pg/ml	0.052	0.0480
S1	60pg/ml	1.999	1.9865
S1	60pg/ml	1.974	1.9865
S2	40pg/ml	1.302	1.3115
S2	40pg/ml	1.321	1.3115
S3	20pg/ml	0.687	0.6900
S3	20pg/ml	0.693	0.6900
S4	10pg/ml	0.392	0.3855
S4	10pg/ml	0.379	0.3855
S5	5pg/ml	0.202	0.2030
S5	5pg/ml	0.204	0.2030