Summary:

Purpose

Our Quick Step Hamster Apolipoprotein B (APO-B) ELISA Kit is to assay APO-B levels in Hamster serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an Antibody specific to APO-B. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific Antibody. Then a Horseradish Peroxidase (HRP)- conjugated Antibody specific for APO-B is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain APO-B and HRP conjugated APO-B Antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of APO-B. You can calculate the concentration of APO-B in the samples by comparing the OD of the samples to the standard curve.

Typical Data

The standard curve of QS0022Ha is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Standard	Concentration	OD Value	Average OD Value
Blank Well	0ng/ml	0.059	0.0560
Blank Well	0ng/ml	0.053	0.0560
S1	360ng/ml	1.975	1.9695
S1	360ng/ml	1.964	1.9695
S2	240ng/ml	1.388	1.3800
S2	240ng/ml	1.372	1.3800
S3	120ng/ml	0.705	0.6995
S3	120ng/ml	0.694	0.6995
S4	60ng/ml	0.383	0.3800
S4	60ng/ml	0.377	0.3800
S5	30ng/ml	0.197	0.2010
S5	30ng/ml	0.205	0.2010

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)