SCC-25 Cell-Specialized Culture Medium - Basic Information

Culture Medium	DMEM-H/F-12 + 10% FBS + 400ng/mL Hydrocortisone
Storage Conditions	Store at 2-8°C, valid for one month
Appearance	Clear, transparent, pale yellow
Procedure	① Preparation: Expose pipettes, 50mL centrifuge tubes, 500mL serum bottles, and the biosafety cabinet to UV light for 20 minutes; pre-warm the basal medium in a 37°C water bath. ② Formulation: Flame-sterilize the openings of the basal medium bottle and serum bottle, then aseptically pour 10% serum and 400ng/mL hydrocortisone into 450mL of basal medium. ③ Sealing: Seal the culture medium bottle opening with sealing film, affix the corresponding product label, and return the medium to a 4°C refrigerator. Wipe the biosafety cabinet with alcohol, extinguish the flame, and UV-irradiate for 30 minutes.