Product Name	Recombinant Rabbit Anti-MACROH2A1 Monoclonal Antibody
Cat No.	SLA1707M
Chinese Name	异型组蛋白H2A.1重组兔单抗
Alias	H2A.y; H2A/y; H2AF12M; H2AFY; MACROH2A1.1; mH2A1; macroH2A1.2; H2AY_HUMAN; MACROH2A1; Histone macroH2A1; Histone H2A.y (H2A/y); Medulloblastoma antigen MU-MB-50.205; H2AY_MOUSE; H2AY_RAT; macroH2A.1 histone; H2A histone family, member Y; H2A histone family member Y
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	4E11
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:200-500) IHC-F (1:200-500) IF (1:200-500) ICC/IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human Histone mH2A1
Purification	affinity purified by Protein A
Predicted MW.	40 kDa
Observed MW.	42 kDa
Gene ID	9555
Swiss-Prot	O75367
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription.Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (macro H2A.1) monoclonal Antibody, unconjugated (SLA1707M) 1:200, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, SL60295G-BF488) at 37°C for 90 min, then stained with Phalloidin/BF555 for 30 min at room temperature; DAPI (blue, C02-04002) was used to stain the cell nuclei.	Figure 2. 4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (macro H2A.1) monoclonal Antibody, unconjugated (SLA1707M) 1:200, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, SL60295G-BF488) at 37°C for 90 min; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Figure 3. 25 ug total protein per lane of various lysates (see on figure) probed with macro H2A.1 monoclonal antibody, unconjugated (SLA1707M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with macro H2A.1 Monoclonal Antibody, Unconjugated (SLA1707M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with macro H2A.1 Monoclonal Antibody, Unconjugated (SLA1707M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with macro H2A.1 Monoclonal Antibody, Unconjugated (SLA1707M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 7. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with macro H2A.1 Monoclonal Antibody, Unconjugated (SLA1707M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 8. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with macro H2A.1 Monoclonal Antibody, Unconjugated (SLA1707M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 9. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with macro H2A.1 Monoclonal Antibody, Unconjugated (SLA1707M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 10. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with macro H2A.1 Monoclonal Antibody, Unconjugated (SLA1707M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 11. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with macro H2A.1 Monoclonal Antibody, Unconjugated (SLA1707M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 12. 4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (macro H2A.1) monoclonal Antibody, unconjugated (SLA1707M) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, SL60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.