Product Name	ERBB2 | HER2/c-ErbB-2/CD340 Rabbit pAb
Cat No.	SL42263R
Chinese Name	ERBB2/HER2抗体
Alias	CD340; HER-2; HER-2/neu; HER2; MLN 19; MLN-19; NEU; NGL; TKR1; VSCN2; c-ERB-2; c-ERB2; p185(erbB2); Erbb-2; c-erbB2; c-neu; l11Jus8; mKIAA3023; HER2/neu; ERBB2_HUMAN; ERBB2; Metastatic lymph node gene 19 protein (MLN 19); Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2; 2.7.10.1; MLN19; ERBB2_MOUSE; Kiaa3023; ERBB2_RAT; Epidermal growth factor receptor-related protein; p185neu; erb-b2 receptor tyrosine kinase 2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog); v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2; neuro/glioblastoma derived oncogene homolog; human epidermal growth factor receptor 2; metastatic lymph node gene 19
Host Species	Rabbit
Isotype	IgG
Clonality	Polyclonal
React Species	Human
Applications	WB (1:500-2000) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	Recombinant human HER2 protein
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	138 kDa
Observed MW.	40 kDa
Subcellular Location	Nucleus
Gene ID	2064
Swiss-Prot	P04626
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008].
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with ERBB2/HER2 polyclonal antibody, unconjugated (SL42263R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.