Product Name	Rabbit Anti-HIF3A Polyclonal Antibody
Cat No.	SLA0070
Chinese Name	缺氧诱导因子3α/HIF-3α抗体
Alias	HIF-3A; HIF3-alpha-1; IPAS; MOP7; PASD7; bHLHe17; HIF3A_HUMAN; HIF3A; HIF-3-alpha; HIF3-alpha; Basic-helix-loop-helix-PAS protein MOP7; Class E basic helix-loop-helix protein 17 (bHLHe17); Inhibitory PAS domain protein (IPAS); Member of PAS protein 7; PAS domain-containing protein 7; hypoxia inducible factor 3 subunit alpha
Host Species	Rabbit
Isotype	IgG
Clonality	Polyclonal
React Species	Human
Applications	WB (1:500-2000) ELISA (1:5000-10000) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	KLH conjugated synthetic peptide derived from human HIF3 alpha
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	74 kDa
Observed MW.	60 kDa
Subcellular Location	Nucleus
Gene ID	64344
Swiss-Prot	Q9Y2N7
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Hypoxia-inducible factor (HIF) is one of the most important factors in the cellular response to hypoxia, transcriptionally activating genes encoding proteins that mediate adaptive responses to reduced oxygen availability. HIF is a heterodimer consisting of one of three subunits, HIF1 alpha, HIF2 alpha, or HIF3 alpha. HIF target genes play critical roles in metabolism, angiogenesis, cell proliferation and cell survival. HIF3 alpha protein is one of several alpha/beta-subunit heterodimeric transcription factors that regulate many adaptive responses to low oxygen tension (hypoxia). The alpha 3 subunit lacks the transactivation domain found in factors containing either the alpha 1 or alpha 2 subunits. HIF3 alpha may be a marker for tumor growth and angiogenesis.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Sample: Lane 1: Human HUVEC cell lysates Lane 2: Human U87MG cell lysates Lane 3: Human SH-SY5Y cell lysates Lane 4: Human HeLa cell lysates Primary: Anti-HIF3 alpha (SLA0070) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 74 kDa Observed band size: 60 kDa

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.