Product Name	Recombinant Rabbit Anti-IL7R Monoclonal Antibody
Cat No.	SLA0569M
Chinese Name	白细胞介素-7受体a（CD127）单克隆抗体
Alias	CD127; CDW127; IL-7R-alpha; IL-7Ralpha; IL7RA; IL7Ralpha; ILRA; IMD104; lnc-IL7R; sIL-7R; IL7RA_HUMAN; IL7R; IL-7 receptor subunit alpha; IL-7R subunit alpha; IL-7RA; IL7RA_MOUSE; interleukin 7 receptor; soluble interleukin-7 receptor; Interleukin-7 receptor subunit alpha
Host Species	Mouse
Clonality	Monoclonal
Clone No.	11D4
React Species	Human
Applications	IHC-P (1:50-200) IHC-F (1:50-200) IF (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	Recombinant human IL-7R Alpha / CD127 protein
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	50 kDa
Subcellular Location	Membrane
Gene ID	3575
Swiss-Prot	P16871
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	The protein encoded by this gene is a receptor for interleukine 7 (IL7). The function of this receptor requires the interleukin 2 receptor, gamma chain (IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukine 2, 4, 7, 9, and 15. This protein has been shown to play a critical role in the V(D)J recombination during lymphocyte development. This protein is also found to control the accessibility of the TCR gamma locus by STAT5 and histone acetylation. Knockout studies in mice suggested that blocking apoptosis is an essential function of this protein during differentiation and activation of T lymphocytes. The functional defects in this protein may be associated with the pathogenesis of the severe combined immunodeficiency (SCID).
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IL7R Monoclonal Antibody, Unconjugated(ascites of SLA0569M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Mouse, SP-0024) and DAB (C-0010) staining.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IL7R Monoclonal Antibody, Unconjugated(ascites of SLA0569M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Mouse, SP-0024) and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IL7R Monoclonal Antibody, Unconjugated(ascites of SLA0569M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Mouse, SP-0024) and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IL7R Monoclonal Antibody, Unconjugated(ascites of SLA0569M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Mouse, SP-0024) and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with IL7R/CD127 Monoclonal Antibody, Unconjugated (SLA0569M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green, SL0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.