Product Name	CD86 | B7-2 Recombinant Rabbit mAb
Cat No.	SLM-43589R
Chinese Name	CD86重组兔单抗
Alias	B7-2; B7.2; B70; CD28LG2; LAB72; CD86_HUMAN; CD86; Activation B7-2 antigen; BU63; CTLA-4 counter-receptor B7.2; FUN-1; CD86 molecule; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen); B-lymphocyte antigen B7-2
Host Species	Rabbit
Isotype	IgG
Clonality	Recombinant
Clone No.	8F6
React Species	Human
Applications	Flow-Cyt (1:50-100) ICC/IF (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	Recombinant human CD86 protein
Purification	affinity purified by Protein A
Concentration
Predicted MW.	37 kDa
Observed MW.
Subcellular Location	Membrane
Gene ID	942
Swiss-Prot	P42081
Storage	Size : 25ul/50ul/100ul/200ul 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Size : 200ug (PBS only) 0.01M PBS (pH7.4). Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011].
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. The Daudi (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-CD86 antibody (SLM-43589R,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed	Figure 2. The MJ (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-CD86 antibody (SLM-43589R,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed
Figure 3. The THP-1 (treated with TPA (80 nM, 18 h), rested in fresh medium for 48 h, and then stimulated with LPS (5 μg/mL, 18 h)) (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-CD86 antibody (SLM-43589R,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed	Figure 4. 4% Paraformaldehyde-fixed Daudi (H) cell; Antibody incubation with (CD86) monoclonal Antibody, unconjugated (SLM-43589R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, SL60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control
Figure 5. 4% Paraformaldehyde-fixed MJ (H) cell; Antibody incubation with (CD86) monoclonal Antibody, unconjugated (SLM-43589R) 1:100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, SL60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.