Product Name	FBLN5 | FIBL-5 Rabbit pAb
Cat No.	SL42060R
Chinese Name	衰老关键蛋白抗体
Alias	ADCL2; ARCL1A; ARMD3; CMT1H; DANCE; EVEC; FIBL-5; HNARMD; UP50; A55; FBLN5_HUMAN; FBLN5; Developmental arteries and neural crest EGF-like protein (Dance); Urine p50 protein (UP50); FBLN5_MOUSE; fibulin 5; embryonic vascular EGF-like repeat-containing protein; developmental arteries and neural crest EGF-like
Host Species	Rabbit
Isotype	IgG
Clonality	Polyclonal
React Species	Human
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) ICC/IF (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	Recombinant human Fibulin 5 protein
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	48 kDa
Observed MW.	30 kDa
Subcellular Location	Secreted
Gene ID	10516
Swiss-Prot	Q9UBX5
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Fibulin 5: A protein that belongs to a family of extracellular proteins expressed in the basement membranes of blood vessels. Fibulin 5 may be essential for the polymerization of elastin. Missense mutations in FBLN5, the gene that encodes fibulin 5, appear responsible for 1-2% of cases of age-related macular degeneration (AMD). FBLN5 is located on chromosome 14 in band 14q32.1. See also: Fibulin 3. May play a role in vascular growth and maturation during development and in lesions of injured vessels.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Sample: Lane 1: Recombinant human Fibulin 5 protein, C-His(SL42060P) Primary: Anti-Fibulin 5 (SL42060R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kDa Observed band size: 30 kDa	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Fibulin 5 Polyclonal Antibody, Unconjugated (SL42060R) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Fibulin 5 Polyclonal Antibody, Unconjugated (SL42060R) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Fibulin 5 Polyclonal Antibody, Unconjugated (SL42060R) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. 4% Paraformaldehyde-fixed WI-38 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Fibulin 5) polyclonal Antibody, unconjugated (SL42060R) 1:100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, SL60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.