Product Name	Rabbit Anti-Xbp1 Polyclonal Antibody
Cat No.	SLA0056
Chinese Name	细胞核转录因子X盒结合蛋白抗体
Alias	TREB-5; TREB5; XBP-1; XBP2; D11Ertd39e; HTF; XBP1_HUMAN; XBP1; Tax-responsive element-binding protein 5 (TREB-5); XBP1_MOUSE; XBP1_RAT; Hepatocarcinogenesis-related transcription factor (HTF); X-box binding protein 1
Host Species	Rabbit
Isotype	IgG
Clonality	Polyclonal
React Species	Mouse,Rat
Applications	WB (1:500-2000) ELISA (1:5000-10000) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	KLH conjugated synthetic peptide derived from mouse XBP1
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	29 kDa
Observed MW.	56 kDa
Gene ID	22433
Swiss-Prot	O35426
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5. [provided by RefSeq, Jul 2008]
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. C2C12 (M) treated with Tunicamycin (2 μg/ml) for 8 h, RAW 264.7 (M) treated with Thapsigargin (300 nM) for 18 h, 25 μg total protein per lane of cell lysates (see on figure) probed with XBP1 polyclonal antibody, unconjugated (SLA0056) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.