Product Name	Rabbit Anti-KSR1 (p-S404) Polyclonal Antibody
Cat No.	SLA0026
Chinese Name	磷酸化Ras信号转导KSR1蛋白抗体
Alias	KSR; RSU2; B-KSR1; D11Bhm183e; D11Bhm184e; hb; mKSR1; KSR1_HUMAN; KSR1; 2.7.11.1; KSR1_MOUSE; Protein Hb; kinase suppressor of ras 1; kinase suppressor of ras; KSR1 (phospho-S404); p-KSR1; phospho-KSR1; KSR1 (phospho-Ser404)
Host Species	Rabbit
Isotype	IgG
Clonality	Polyclonal
React Species	Human
Applications	WB (1:500-2000) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	KLH conjugated synthesised phosphopeptide derived from human KSR1 around the phosphorylation site of Ser404
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	102 kDa
Observed MW.	110 kDa
Subcellular Location	Cytoplasm
Gene ID	8844
Swiss-Prot	Q8IVT5
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	KSR1 is a molecular scaffold and positive regulator of the Raf/MEK/ERK phosphorylation cascade. It was initially considered to be a protein kinase acting in the Ras pathway. KSR1 is required for maximal ERK activation induced by growth factors and by some cytotoxic agents. In resting cells, phosphorylated KSR1 is cytoplasmic and in stimulated cells, dephosphorylated KSR1 is membrane associated.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Hela (H) cells were treated with or without Calyculin A (50nM 30) for 30 min, 25 μg total protein per lane of cell lysates (see on figure) probed with phospho-KSR1 (Ser404) polyclonal antibody, unconjugated (SLA0026) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.