Product Name	Recombinant Rabbit Anti-LPAR1 Monoclonal Antibody
Cat No.	SLA1654M
Chinese Name	溶血磷脂酸受体蛋白1/EDG2重组兔单抗
Alias	EDG2; Gpcr26; LPA1; Mrec1.3; VZG1; edg-2; rec.1.3; vzg-1; Kdt2; LPAR1_BOVIN; LPAR1; LPA receptor 1; LPA-1; Lysophosphatidic acid receptor Edg-2; Rec1.3; LPAR1_HUMAN; LPAR1_MOUSE; LPAR1_RAT; Gpcr91; LPAR1_SHEEP; lysophosphatidic acid receptor 1; endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor, 2
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	7C2
React Species	Human
Applications	IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human LPA receptor 1
Purification	affinity purified by Protein A
Predicted MW.	41 kDa
Observed MW.	39 kDa
Subcellular Location	Cytoplasm
Gene ID	1902
Swiss-Prot	Q92633
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Plays a role in the reorganization of the actin cytoskeleton, cell migration, differentiation and proliferation, and thereby contributes to the responses to tissue damage and infectious agents. Activates downstream signaling cascades via the G(i)/G(o), G12/G13, and G(q) families of heteromeric G proteins. Signaling inhibits adenylyl cyclase activity and decreases cellular cAMP levels.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Paraformaldehyde-fixed, paraffin embedded Human Prostate Tumor; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LPA1 Monoclonal Antibody, Unconjugated (SLA1654M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LPA1 Monoclonal Antibody, Unconjugated (SLA1654M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LPA1 Polyclonal Antibody, Unconjugated (SLA1654M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LPAR1 | LPA1 Antibody, Unconjugated (SLA1654M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, SL0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Prostate Tumor; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LPAR1 | LPA1 Antibody, Unconjugated (SLA1654M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, SL0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.