Product Name	Recombinant Rabbit Anti-CDH16 Monoclonal Antibody
Cat No.	SLA1821M
Chinese Name	钙粘蛋白16重组兔单抗
Alias	CAD16_HUMAN; CDH16; Kidney-specific cadherin (Ksp-cadherin); CAD16_MOUSE; CAD16_RABIT; cadherin 16; cadherin 16, KSP-cadherin
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	12G2
React Species	Human,Mouse
Applications	WB (1:500-2000) IHC-P (1:200-1000) IHC-F (1:200-1000) IF (1:200-1000) IP (1:20-50) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human Cadherin 16
Purification	affinity purified by Protein A
Predicted MW.	90 kDa
Observed MW.	110 kDa
Subcellular Location	Membrane
Gene ID	1014
Swiss-Prot	O75309
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with Cadherin 16 monoclonal antibody, unconjugated (SLA1821M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cadherin 16 Monoclonal Antibody, Unconjugated(SLA1821M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cadherin 16 Monoclonal Antibody, Unconjugated(SLA1821M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CDH16 | Ksp-cadherin Antibody, Unconjugated (SLA1821M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Green, SL0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CDH16 | Ksp-cadherin Antibody, Unconjugated (SLA1821M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Green, SL0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.