Product Name	Recombinant Rabbit Anti-Aif1 Monoclonal Antibody
Cat No.	SLA0545M
Chinese Name	离子钙接头蛋白重组兔单抗
Alias	AIF-1; IBA1; IRT-1; IRT1; D17H6S50E; G1; BART-1; Bart1; mrf-1; AIF1_HUMAN; AIF1; Ionized calcium-binding adapter molecule 1; Protein G1; AIF1_MOUSE; AIF1_RAT; Microglia response factor; Mrf1; allograft inflammatory factor 1; interferon gamma responsive transcript
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	2C8
React Species	Mouse
Applications	WB (1:500-2000) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	Recombinant mouse AIF1 protein
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	16 kDa
Observed MW.	15 kDa
Subcellular Location	Cytoplasm
Gene ID	11629
Swiss-Prot	O70200
Storage	Size : 25ul/50ul/100ul/200ul 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Size : 200ug (PBS only) 0.01M PBS (pH7.4). Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Allograft Inflammatory Factor-1 (AIF1)or ionized calcium-binding adaptor molecule 1 (Iba1) is expressed selectively in microglia/macrophages and is a Ca2+-binding peptide produced by activated monocytes and microglial cells. It has been suggested that AIF1 expression is associated with chronic inflammatory processes. AIF1 is expressed by activated monocytes and might participate in a variety of pathogenic processes in the mammalian brain and in chronic transplant rejection. It has been shown to be expressed early and persistently in chronically rejecting cardiac allografts but not in cardiac syngrafts and host hearts.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with AIF1 monoclonal antibody, unconjugated (SLA0545M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.