Product Name	Recombinant Rabbit Anti-NOS2 Monoclonal Antibody
Cat No.	SLA1018M
Chinese Name	一氧化氮合成酶-2重组兔单抗
Alias	HEP-NOS; INOS; NOS; NOS2A; MAC-NOS; NOS-II; Nos-2; i-NOS; NOS2_HUMAN; NOS2; Hepatocyte NOS (HEP-NOS); Inducible NO synthase (Inducible NOS | iNOS); NOS type II; Peptidyl-cysteine S-nitrosylase NOS2; 1.14.13.39; NOS2_MOUSE; Macrophage NOS (MAC-NOS); Inosl; NOS2_RAT; nitric oxide synthase 2; nitric oxide synthase 2A (inducible, hepatocytes); nitric oxide synthase 2, inducible
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	10H1
React Species	Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:50-200) IHC-F (1:50-200) IF (1:50-200) ICC/IF (1:50-200) ELISA (1:5000-10000) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	KLH conjugated synthetic peptide derived from human NOS-2
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	130 kDa
Subcellular Location	Cytoplasm
Gene ID	4843
Swiss-Prot	P35228
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. Nitric oxide synthase is expressed in liver, macrophages, hepatocytes, synoviocytes, stimulated glial cells and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time. Human iNOS is regulated by calcium/calmodulin (in contrast with mouse NOS2).
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.