Product Name	Recombinant Rabbit Anti-NCAM1 Monoclonal Antibody
Cat No.	SLA0965M
Chinese Name	神经细胞粘附分子1重组兔单抗
Alias	CD56; MSK39; NCAM; E-NCAM; NCAM-1; N-CAM; N-CAM-1; NCAM-C; NCAMC; NCAM1_HUMAN; NCAM1; NCAM1_MOUSE; NCAM1_RAT; neural cell adhesion molecule 1
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	4E9
React Species	Human
Applications	ICC/IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	KLH conjugated synthetic peptide derived from human NCAM
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	92 kDa
Subcellular Location	Membrane
Gene ID	4684
Swiss-Prot	P13591
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2011]
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 4% Paraformaldehyde-fixed SH-SY5Y (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (NCAM) monoclonal Antibody, unconjugated (SLA0965M) 1:100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, SL60295G-BF488) at 37°C for 90 min, then stained with Phalloidin/BF555 for 30 min at room temperature; DAPI (blue, C02-04002) was used to stain the cell nuclei.

Figure 2. 4% Paraformaldehyde-fixed SH-SY5Y (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (NCAM) monoclonal Antibody, unconjugated (SLA0965M) 1:100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, SL60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.