Product Name	Rabbit Anti-NEFL Polyclonal Antibody
Cat No.	SLA0253
Chinese Name	低分子量神经丝蛋白抗体
Alias	CMT1F; CMT2E; CMTDIG; NF-L; NF68; NFL; PPP1R110; NFL_HUMAN; NEFL; 68 kDa neurofilament protein; Neurofilament triplet L protein; NFL_MOUSE; NFL_RAT; neurofilament light chain; neurofilament, light polypeptide 68kDa; neurofilament, light polypeptide; neurofilament light; protein phosphatase 1, regulatory subunit 110
Host Species	Rabbit
Isotype	IgG
Clonality	Polyclonal
Clone No.
React Species	Human,Mouse
Applications	WB (1:500-2000) Flow-Cyt (1:100-200) ELISA (1:5000-10000) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	Recombinant human NF-L protein
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	61 kDa
Observed MW.	65 kDa
Subcellular Location	Cytoplasm
Gene ID	4747
Swiss-Prot	P07196
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Neurofilament light polypeptide also called NF-L; Neurofilament triplet L protein; 68 kDa neurofilament protein. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions. NF-L is the most abundant of the three neurofilament proteins and, as the other nonepithelial intermediate filament proteins, it can form homopolymeric 10-nm filaments. Belongs to the intermediate filament family. Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-L is the light or low molecular weight microfilament subunit and runs on SDS-PAGE gels at approximately 70 kDa. Neurofilament are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels in the range 180-220 kDa, with some variation in different species.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with NF-L polyclonal antibody, unconjugated (SLA0253) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min Figure 2. The SK-N-BE (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Cells stained with Primary Antibody for 30 min at room temperature,followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-NF-L antibody (SLA0253,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.