Product Name	Rabbit Anti-MAP2 Polyclonal Antibody
Cat No.	SLA0131
Chinese Name	微管相关蛋白2抗体
Alias	MAP-2; MAP2A; MAP2B; MAP2C; G1-397-34; Mtap-2; Mtap2; repro4; MAP2R; MTAP2_HUMAN; MAP2; MTAP2_MOUSE; MTAP2_RAT; microtubule associated protein 2
Host Species	Rabbit
Isotype	IgG
Clonality	Polyclonal
React Species	Human,Mouse,Rat
Applications	IHC-P (1:300-1500) IHC-F (1:300-1500) IF (1:300-1500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	KLH conjugated synthetic peptide derived from human MAP2
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	199 kDa
Subcellular Location	Nucleus
Gene ID	4133
Swiss-Prot	P11137
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	MAP2 is the major microtubule associated protein of brain tissue. There are three forms of MAP2; two are similarily sized with apparent molecular weights of 280 kDa (MAP2a and MAP2b) and the third with a lower molecular weight of 70 kDa (MAP2c). In the newborn rat brain, MAP2b and MAP2c are present, while MAP2a is absent. Between postnatal days 10 and 20, MAP2a appears. At the same time, the level of MAP2c drops by 10-fold. This change happens during the period when dendrite growth is completed and when neurons have reached their mature morphology. MAP2 is degraded by a Cathepsin D-like protease in the brain of aged rats. There is some indication that MAP2 is expressed at higher levels in some types of neurons than in other types. MAP2 is known to promote microtubule assembly and to form side-arms on microtubules. It also interacts with neurofilaments, actin, and other elements of the cytoskeleton.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MAP2 Polyclonal Antibody, Unconjugated (SLA0131) at 1:1500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MAP2 Polyclonal Antibody, Unconjugated (SLA0131) at 1:1500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MAP2 Polyclonal Antibody, Unconjugated (SLA0131) at 1:1500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MAP2 Polyclonal Antibody, Unconjugated (SLA0131) at 1:1500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MAP2 Polyclonal Antibody, Unconjugated (SLA0131) at 1:1500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.