Product Name	Recombinant Rabbit Anti-CD69 Monoclonal Antibody
Cat No.	SLA0619M
Chinese Name	活化诱导分子CD69单克隆抗体
Alias	AIM; BL-AC/P26; CLEC2C; EA1; GP32/28; MLR-3; 5830438K24Rik; VEA; CD69_HUMAN; CD69; Activation inducer molecule (AIM); C-type lectin domain family 2 member C; Early T-cell activation antigen p60; Leukocyte surface antigen Leu-23; CD69_MOUSE; CD69 molecule; CD69 antigen (p60, early T-cell activation antigen)
Host Species	Mouse
Isotype	IgG
Clonality	Monoclonal
Clone No.
React Species	Human
Applications	Flow-Cyt (1:100-200) ICC/IF (1:100-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	Recombinant human CD69 protein
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	22 kDa
Observed MW.
Subcellular Location	N/A
Gene ID	969
Swiss-Prot	Q07108
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	This gene encodes a member of the calcium dependent lectin superfamily of type II transmembrane receptors. Expression of the encoded protein is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. [provided by RefSeq, Aug 2011]
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. The Daudi(H, treated with 50 ng/ml PMA for 24h) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Cells stained with Primary Antibody for 30 min at room temperature, followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Mouse Anti-CD69 antibody (SLA0619M,1:100); Isotype Control (orange): Mouse IgG (SL0296P). Blank control (black): PBS. Acquisition of 20,000 events was performed Figure 2. 4% Paraformaldehyde-fixed Daudi treated with 50 ng/ml PMA for 24h(M) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (CD69) monoclonal Antibody, unconjugated (SLA0619M) 1:100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Mouse IgG antibody (green, SL60296G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.

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