Product Name	Rabbit Anti-ADAMTS13 Polyclonal Antibody
Cat No.	SLA0213
Chinese Name	整合素样金属蛋白酶与凝血酶13型抗体
Alias	ADAM-TS13; ADAMTS-13; C9orf8; VWFCP; vWF-CP; Gm710; ATS13_HUMAN; ADAMTS13; ADAM-TS 13; von Willebrand factor-cleaving protease (vWF-CP | vWF-cleaving protease); 3.4.24.87; ATS13_MOUSE; ADAM metallopeptidase with thrombospondin type 1 motif 13; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 13
Host Species	Rabbit
Isotype	IgG
Clonality	Polyclonal
Clone No.
React Species	Human
Applications	Flow-Cyt (1:100-200) ICC/IF (1:100-200) ELISA (1:5000-10000) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	Recombinant human ADAMTS13 protein
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	145 kDa
Observed MW.	160 kDa
Subcellular Location	Secreted
Gene ID	11093
Swiss-Prot	Q76LX8
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motif) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene is the von Willebrand Factor (vWF)-cleaving protease, which is responsible for cleaving at the site of Tyr842-Met843 of the vWF molecule. A deficiency of this enzyme is associated with thrombotic thrombocytopenic purpura. Alternative splicing of this gene generates multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2008].
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. The A549 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-ADAMTS13 antibody (SLA0213): 1 μg/10^6 cells; Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed Figure 2. 4% Paraformaldehyde-fixed A549 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (ADAMTS13) polyclonal Antibody, unconjugated (SLA0213) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, SL60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.

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