Product Name	Recombinant Rabbit Anti-SPTA1 Monoclonal Antibody
Cat No.	SLA2848M
Chinese Name	血影蛋白A链红细胞型重组兔单抗
Alias	EL2; HPP; HS3; SPH3; SPTA; EL3; HS2; HSPTB1; SPH2; Spna-1; Spna1; ha; ihj; nmf4; sph; D330027P03Rik; Gm1301; Spnb-1; Spnb1; ja; jaundiced; mKIAA4219; SPTA1_HUMAN; SPTA1; Erythroid alpha-spectrin; SPTB1_HUMAN; SPTB; Beta-I spectrin; SPTB1; SPTA1_MOUSE; SPTB1_MOUSE; spectrin alpha, erythrocytic 1; spectrin, alpha, erythrocytic 1 (elliptocytosis 2); elliptocytosis 2
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	3C10
React Species	Human,Mouse
Applications	WB (1:500-2000) Flow-Cyt (1:100-200) ICC/IF (1:100-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human Spectrin alpha chain
Purification	affinity purified by Protein A
Predicted MW.	280 kDa
Observed MW.	300 kDa
Subcellular Location	Cytoplasm
Gene ID	6708
Swiss-Prot	P02549
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with Spectrin alpha chain monoclonal antibody, unconjugated (SLA2848M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min Figure 2. The K562 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-Spectrin alpha chain antibody (SLA2848M,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.