DESCRIPTION

Triggering receptor expressed on myeloid cells-1 (TREM-1, also known as CD354) is a member of the immunoglobulin superfamily. It plays a crucial role in the innate immune response to bacterial and fungal infections by triggering the release of pro-inflammatory chemokines and cytokines and increasing the surface expression of cell activation markers. TREM-1 also exists as a soluble receptor (sTREM-1) that arises from proteolytic cleavage of the membrane-bound receptor by matrix metalloproteases. The serum levels of sTREM-1 may correlate with disease severity and treatment outcomes in pulmonary tuberculosis patients.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	246.2	12.8	5.2
2	8	60.7	2.0	3.3
3	8	32.2	1.7	5.2

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	253.6	14.0	5.5
2	16	60.7	2.9	4.8
3	16	31.0	2.0	6.4

RECOVERY

The recovery of human TREM-1/CD354 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Human serum	1:8	79	76-82
	1:16	80	75-86
Cell culture supernatant	1:2	106	105-107
	1:4	103	101-104
Saliva	1:8	107	104-109
	1:16	105	101-110
Cell lysate	1:8	106	93-118
	1:16	101	77-122

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay.

		Human serum	Cell culture supernatant	Saliva	Cell lysate
1:2	Average% of Expected	100	100	100	100
	Range (%)	-	-	-	-
1:4	Average% of Expected	101	97	102	79
	Range (%)	95-107	92-103	87-112	76-82
1:8	Average% of Expected	103	94	104	81
	Range (%)	92-117	93-96	91-112	80-83
1:16	Average% of Expected	103	83	104	104
	Range (%)	89-111	74-91	89-117	89-118

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human TREM-1/CD354 Sandwich ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)