DESCRIPTION

TP53BP1 (Tumor Protein P53 Binding Protein 1) is a key protein involved in the DNA damage response (DDR) and repair mechanisms, particularly in the non-homologous end joining (NHEJ) pathway. It plays a crucial role in maintaining genomic stability and has implications in cancer development and treatment. TP53BP1 is ubiquitously expressed in various tissues, including the brain and testis. Its expression levels are significantly higher in some kinds of cancerous tissues, for instance, higher levels of TP53BP1 were observed in non-small cell lung cancer, skin cancer, and cervical cancer specimens.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	8	31.87	1.95	6.10
2	8	7.07	0.32	4.50
3	8	3.68	0.12	3.40

Inter-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	16	32.29	1.54	4.77
2	16	6.85	0.32	4.72
3	16	3.61	0.13	3.59

RECOVERY

The recovery of human TP53BP1 spiked to three different levels throughout the range of the assay in cell lysate was evaluated.

Sample Type		Average% of Expected	Range (%)
Cell lysate	1:8	91	83-96
	1:16	93	91-96

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay.

		Cell lysate
1:2	Average% of Expected	100
	Range (%)	-
1:4	Average% of Expected	112
	Range (%)	109-114
1:8	Average% of Expected	118
	Range (%)	115-121
1:16	Average% of Expected	113
	Range (%)	110-116

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human TP53BP1 Sandwich ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)