DESCRIPTION

TREM2 (triggering receptor expressed on myeloid cells 2) is a cell surface receptor belongs to TREM family that is expressed on osteoclast, dendritic cells, macrophages, nature killers, neutrophils and microglia. TREM2 is localized predominantly in the Golgi complex, but also shuttles to and from the cell surface in endocytic and exocytic vesicles. It associates with DAP12 to initiate the intracellular signalling cascade via an immunoreceptor tyrosine-based activation motif (ITAM) domain and tyrosine-kinases.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 20 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 24 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	20	800.0	31.4	3.9
2	20	179.3	8.1	4.5
3	20	40.4	3.8	9.5

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	24	719.9	43.5	6.0
2	24	167.8	7.3	4.3
3	24	45.4	4.1	9.1

RECOVERY

The recovery of human TREM2 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Human serum	1:8	106	95-125
	1:16	100	91-120
Cell culture supernatant	1:8	85	74-101
	1:16	91	78-104
Cell lysate	1:32	83	77-89
	1:64	84	74-96
Human cerebrospinal fluid (CSF)	1:32	85	78-93
	1:64	95	85-110

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The cell lysate was initially diluted 1:4. The CSF was initially diluted 1:4.)

		human serum (SampleDiluent PT 3)	Cell culturesupernatant(SampleDiluent PT 4B1)	Cell lysate(SampleDiluent PT 4B1)	Humancerebrospinal fluid(CSF) (SampleDiluent PT 4B1)
1:2	Average% of Expected	100	100	100	100
	Range (%)	-	-	-	-
1:4	Average% of Expected	110	105	88	107
	Range (%)	105-115	100-111	84-92	102-111
1:8	Average% of Expected	124	103	87	106
	Range (%)	120-128	94-112	82-92	105-107
1:16	Average% of Expected	122	108	96	121
	Range (%)	121-123	91-125	89-103	113-130

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human TREM2 produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
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• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)