Product Name	Recombinant Rabbit Anti-Myo7a Monoclonal Antibody
Cat No.	SLA0551M
Chinese Name	肌球蛋白7a/常染色体隐性耳聋蛋白2重组兔单抗
Alias	MYO7A_HUMAN; MYO7A; USH1B; DFNA11; DFNB2; MYOVIIA; MYU7A; NSRD2; myosin VIIA; myosin VIIA (Usher syndrome 1B (autosomal recessive, severe))
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	10H4
React Species	Mouse
Applications	WB (1:500-2000) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	Recombinant mouse Myosin VIIa protein
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	244 kDa
Observed MW.	255 kDa
Subcellular Location	Cytoplasm
Storage	Size : 25ul/50ul/100ul/200ul 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Size : 200ug (PBS only) 0.01M PBS (pH7.4). Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments. In retina, myosin VIIa may play a role in trafficking of ribbon-synaptic vesicle complexes and renewal of the outer photoreceptors disks. In inner ear, it may maintain the rigidity of stereocilia during the dynamic movements of the bundle.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Sample: Lane 1: Recombinant mouse Myosin VIIa protein, N-Trx-His(SL42234P) Primary: Anti-Myosin VIIa (SLA0551M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 244 kDa Observed band size: 42 kDa

Figure 2. 25 ug total protein per lane of various lysates (see on figure) probed with Myosin VIIa monoclonal antibody, unconjugated (SLA0551M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.