Product Name	Recombinant Rabbit Anti-FGF21 Monoclonal Antibody
Cat No.	SLA0870M
Chinese Name	成纤维细胞生长因子21重组兔单抗
Alias	Fgf8c; FGF21_HUMAN; FGF21; FGF-21; FGF21_MOUSE; fibroblast growth factor 21
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	7C4
React Species	Human,Mouse,Rat
Applications	WB (1:1000-5000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human FGF21
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	20 kDa
Observed MW.	22 kDa
Subcellular Location	Secreted
Gene ID	26291
Swiss-Prot	Q9NSA1
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	FGF21 is a growth factor that is a member of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF21, in the presence of betaKlotho as a protein cofactor, signals through the FGFR 1c and 4 receptors and stimulates insulin independent glucose uptake by adipocytes.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with FGF21 monoclonal antibody, unconjugated (SLA0870M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with FGF21 Monoclonal Antibody, Unconjugated(SLA0870M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with FGF21 Monoclonal Antibody, Unconjugated(SLA0870M) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with FGF21 Monoclonal Antibody, Unconjugated(SLA0870M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with FGF21 Monoclonal Antibody, Unconjugated(SLA0870M) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FGF21 Monoclonal Antibody, Unconjugated (SLA0870M) at 1:200 overnight at 4°C, followed by conjugation to the PV-1023 and DAB (C-0010) staining.
Figure 7. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FGF21 Monoclonal Antibody, Unconjugated (SLA0870M) at 1:200 overnight at 4°C, followed by conjugation to the PV-1023 and DAB (C-0010) staining.	Figure 8. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FGF21 Monoclonal Antibody, Unconjugated (SLA0870M) at 1:200 overnight at 4°C, followed by conjugation to the PV-1023 and DAB (C-0010) staining.
Figure 9. Paraformaldehyde-fixed, paraffin embedded Human Pancreatic Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FGF21 Monoclonal Antibody, Unconjugated (SLA0870M) at 1:200 overnight at 4°C, followed by conjugation to the PV-1023 and DAB (C-0010) staining.	Figure 10. Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FGF21 Antibody, Unconjugated (SLA0870M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, SL0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.