Product Name	Rabbit Anti-NOS3 Polyclonal Antibody
Cat No.	SLA0096
Chinese Name	一氧化氮合成酶-3（内皮型）抗体
Alias	ECNOS; eNOS; 2310065A03Rik; Nos-3; NOS3_BOVIN; NOS3; Constitutive NOS (cNOS); EC-NOS; NOS type III (NOSIII); Nitric oxide synthase, endothelial (Endothelial NOS | eNOS); 1.14.13.39; NOS3_HUMAN; NOS3_MOUSE; NOS3_RAT; nitric oxide synthase 3; nitric oxide synthase 3 (endothelial cell); endothelial nitric oxide synthase
Host Species	Rabbit
Isotype	IgG
Clonality	Polyclonal
React Species	Human
Applications	IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	KLH conjugated synthetic peptide derived from human eNOS
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	133 kDa
Subcellular Location	Cytoplasm
Gene ID	4846
Swiss-Prot	P29474
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Nitric oxide synthase NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation neurotransmission, anti tumor and anti pathogenic activities. NOS is classified under three types: neuronal NOS (nNOS) or brain NOS (bNOS); inducible NOS (iNOS) or macrophage NOS (mNOS); and endothelial NOS (eNOS).
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (eNOS) Polyclonal Antibody, Unconjugated (SLA0096) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Figure 2. Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (eNOS) Polyclonal Antibody, Unconjugated (SLA0096) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.