Product Name	Rabbit Anti-PPP3CA Polyclonal Antibody
Cat No.	SLA0325
Chinese Name	钙调磷酸酶A/钙依赖磷酸酶A抗体
Alias	ACCIID; CALN; CALNA; CALNA1; CCN1; CNA1; DEE91; IECEE; IECEE1; PPP2B; 2900074D19Rik; CN; CnA; PP2BA_HUMAN; PPP3CA; CAM-PRP catalytic subunit; Calcineurin A alpha; Calmodulin-dependent calcineurin A subunit alpha isoform (CNA alpha); Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform; 3.1.3.16; PP2BA_MOUSE; PP2BA_RAT; protein phosphatase 3 catalytic subunit alpha; protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform (calcineurin A alpha); protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform; protein phosphatase 3, catalytic subunit, alpha isozyme; protein phosphatase 2B, catalytic subunit, alpha isoform
Host Species	Rabbit
Isotype	IgG
Clonality	Polyclonal
React Species	Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	KLH conjugated synthetic peptide derived from human PPP3CA
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	59 kDa
Observed MW.	57 kDa
Subcellular Location	Nucleus
Gene ID	5530
Swiss-Prot	Q08209
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Calcineurin, a major soluble calmodulin binding protein in the brain, a Ca2+/calmodulin dependent serine/threonine protein phosphatase, with a relatively narrow substrate specificity. This metalloenzyme, also known as phosphatase 2B, is a heterodimer composed of a calmodulin binding, catalytic alpha subunit 61 kDa, calcineurin A) and calcium binding beta subunit (18 kDa, calcineurin B). The Ca2+ binding subunit, calcineurin B, is immunologically conserved from yeast to mammals. The presence of 2 different calcineurin B isoforms (beta 1 and beta 2) has been reported in rat testis. The catalytic subunit of calcineurin, calcineurin A, isolated from different tissues or different organisms, exhibits some immunological heterogeneity. Further, there are at least 2 isoforms of calcineurin A in bovine brain (alpha 1 and alpha 2, 61 and 59 kDa, respectively).
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with PPP3CA polyclonal antibody, unconjugated (SLA0325) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Figure 2. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PPP3CA) Polyclonal Antibody, Unconjugated (SLA0325) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.